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 CYMBAL SABIAN 20"/51CM 87.93 OZ STAGE RIDE AAX SERIES...GC...GUARANTEED!!! US $149.99 
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 Sabian AAX Series 20'' O-Zone Crash Cymbal New US $224.99
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 Sabian 18" AAX Series Stage Crash NEVER HIT! US $215.00
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 Mapex 5 Piece M Series Blue Drumset W/ Full Hardware, AAX Sabian Ride, B8 Hi Hat US $449.00
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 Sabian AAX Series Studio Crash Cymbal 16 Inches US $195.00
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 Sabian AAX Series Metal Crash Cymbal 20 Inches US $265.00
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 Sabian AAX Series Stage Hi-Hat Cymbals 13 Inches US $289.00
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 Sabian AAX Series Dark Crash Cymbal 15 Inches US $175.00
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 Sabian AAX Series Cymbal Effects Pack/Set - 25005XE US $329.00
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 Sabian AAX Studio Series 14" Crash Cymbal - 21406X US $159.00
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 Sabian AAX Dark Series 14" Dark Crash Cymbal - 21468X US $159.00
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 Sabian AAX Studio Series 15" Crash Cymbal - 21506X US $175.00
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 Sabian AAX Dark Series 15" Dark Crash Cymbal - 21568X US $175.00
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 Sabian AAX X-Plosion Series 15" Crash Cymbal - 21587XB US $175.00
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 Sabian AAX Series 16" O-Zone Crash Cymbal - 21600X US $195.00
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 Sabian AAX Studio Series 16" Crash Cymbal - 21606X US $195.00
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 Sabian AAX Stage Series 16" Crash Cymbal - 21608X US $195.00
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 Sabian AAX Metal Series 16" Crash Cymbal - 21609X US $195.00
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 Sabian AAX Bright Series 16" Crash Cymbal - 21637X US $195.00
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 Sabian AAX Dark Series 16" Dark Crash Cymbal - 21668X US $195.00
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 Sabian AAX X-Plosion Series 16" Crash Cymbal - 21687XB US $195.00
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 Sabian AAX Studio Series 17" Crash Cymbal - 21706X US $209.00
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 Sabian AAX Dark Series 17" Dark Crash Cymbal - 21768X US $209.00
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 Sabian AAX Series 18" O-Zone Crash Cymbal - 21800X (Natural) US $225.00
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 Sabian AAX Studio Series 18" Crash Cymbal - 21806X (Natural) US $225.00
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 Sabian AAX X-Plosion Series 17" Crash Cymbal - 21787XB (Brilliant) US $209.00
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 Sabian AAX Stage Series 18" Crash Cymbal - 21808X (Natural) US $225.00
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 Sabian AAX Metal Series 18" Crash Cymbal - 21809X (Natural) US $225.00
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 Sabian AAX Bright Series 18" Crash Cymbal - 21837X (Natural) US $225.00
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 Sabian AAX Dark Series 18" Dark Crash Cymbal - 21868X (Natural) US $225.00
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 Sabian AAX X-Plosion Series 18" Crash Cymbal - 21887XB US $225.00
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 Sabian AAX Stage Series 19" Crash Cymbal - 21908X (Natural) US $239.00
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 Sabian AAX Metal Series 19" Crash Cymbal - 21909X US $239.00
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 Sabian AAX X-Plosion Series 19" Crash Cymbal - 21987XB US $239.00
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 Sabian AAX Series 20" O-Zone Crash Cymbal - 22000X US $265.00
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 Sabian AAX Metal Series 20" Crash Cymbal - 22009X US $265.00
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 Sabian AAX X-Plosion Series 20" Crash Cymbal - 22087XB US $265.00
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 Sabian AAX Studio Series 20" Ride Cymbal - 22010X US $265.00
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 Sabian AAX Stage Series 20" Ride Cymbal - 22012X US $265.00
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 Sabian AAX Metal Series 20" Ride Cymbal - 22014X US $265.00
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 Sabian AAX Dry Series 20" Dry Ride Cymbal - 22035X (Natural) US $265.00
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 Sabian AAX Series 21" Memphis Ride Cymbal - 221101X US $289.00
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 Sabian AAX Stage Series 21" Ride Cymbal - 22112X US $289.00
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 Sabian AAX Series 21" Raw Bell Dry Ride Cymbal - 22172X US $289.00
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 Sabian AAX Series 6" Splash Cymbal - 20605X US $79.00
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 Sabian AAX Series 8" Splash Cymbal - 20805X US $99.00
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 Sabian AAX Series 10" Splash Cymbal - 21005X US $119.00
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 Sabian AAX X-Plosion Series 11" Splash Cymbal - 21187XB US $125.00
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 Sabian AAX Series 12" Splash Cymbal - 21205X US $135.00
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 Sabian AAX Series 12" Metal Splash Cymbal - 21209X US $135.00
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 Sabian AAX Series 12" Mini Chinese Cymbal - 21216X US $135.00
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 Sabian AAX Series 14" Mini-Chinese Cymbal - 21416X US $159.00
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 Sabian AAX Series 16" Chinese Cymbal - 21616X US $195.00
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 Sabian AAX X-Treme Series 15" Chinese Cymbal - 21586X US $175.00
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 Sabian AAX X-Treme Series 17" Chinese Cymbal - 21786X (Natural) US $209.00
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 Sabian AAX Series 18" Chinese Crash Cymbal - 21816X (Natural) US $225.00
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 Sabian AAX X-Treme Series 19" Chinese Cymbal - 21986X US $239.00
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 Sabian AAX Series 20" Chinese Cymbal - 22016X US $265.00
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 Sabian AAX Series 14" Inch Stage Hi-Hat Cymbals--Top and Bottom 189157 US $127.96
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 Sabian AAX Series Stage Crash Cymbal 20" US $265.00
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 Sabian AAX Series Studio Ride 20 Inches US $265.00
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 Sabian AAX Series Dark Crash Cymbal 20" US $279.00
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 Sabian AAX Series Metal Ride Cymbal 20 Inches US $265.00
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 Sabian AAX Series Metal Ride Cymbal US $265.00
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 Sabian AAX Series Stage Crash Cymbal 17 Inches US $209.00
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 Sabian AAX Series Studio Ride 20" US $265.00
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 Sabian AAX Series Stage Ride Cymbal 21 Inches US $289.00
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 Sabian AAX Series Dark Crash Cymbal 17 Inches US $209.00
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 Sabian AAX Series Dark Crash Cymbal 17 Inches US $209.00
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 Sabian AAX Series Studio Crash Cymbal 14 Inches US $159.00
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 Sabian AAX Series Studio Crash Cymbal 13 Inches US $145.00
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 SABIAN 21609X AAX Series 16" Metal Crash Cymbal 16 inch US $195.00
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 Sabian 21887XB AAX Series 18" AAXplosion Crash Cymbal - Brilliant Finish US $239.00
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 Sabian 21587XB AAX Series 15" AAXplosion Crash Cymbal US $185.00
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 Sabian 221101X AAX Series 21" Memphis Ride Cymbal - Natural Finish US $299.00
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 Sabian AAX Series Studio Crash Cymbal 17 Inches US $209.00
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 Sabian 21786X 17" AAX Series AAXtreme Chinese Thin Cymbal US $169.00
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 Sabian AAX Series Metal Ride Cymbal 22 Inches US $315.00
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 Sabian B8 Splash Autograph Series cymbal 8" Brilliant New AAX style lathing US $25.99
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 Sabian AAX Series Stage Ride Cymbal 21 Inches US $289.00
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 SABIAN AAX Series Dark Crash 19" Cymbal - 21968X REGULAR OR BRILLIAN FINISH US $239.00
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 Sabian AAX Series Dark Crash Cymbal 16 Inches US $195.00
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 Sabian AAX Series Stage Crash Cymbal 16 Inches US $195.00
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 Sabian AAX Series Stage Crash Cymbal 18 Inches US $225.00
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Aax Series
Sabian Cymbals AAX Modern Bright Series
S-Farnesylated Cysteine Peptides
Definition
RAS and many other oncogenic proteins undergo a complex series of post-translational modifications that are initiated by the addition of farnesyl or a geranyl-geranyl moiety to C-terminal cysteine(s) of the target protein through a process known as prenylation.
Discovery
Fujiyama A in 1991 demonstrate that polyisoprenylation and methyl esterification of the cysteine residue in the C-terminal domain of the RAS2 protein are involved in the conversion process from precursor form to intermediate form. The polyisoprenoid moiety attached to the RAS2 protein was identified as a 15-carbon farnesyl group through two independent experiments: the release of S-farnesylcysteine with carboxypeptidase Y from the RAS2 protein, and the recovery of radioactive farnesol through methyliodide treatment of the RAS2 protein purified. The farnesyl group attached to the RAS2 protein was detected predominantly in the C-terminal peptide 1. The mam4 mutation of Schizosaccharomyces pombe causes mating deficiency in h2 cells but not in h1 cells. h2 cells defective in mam4 do not secrete active mating pheromone M-factor. Xmam4 was shown to have a farnesyl cysteine carboxyl methyltransferase activity in S. pombe cells 2.
Structural Characteristics
Eukaryotic polypeptides synthesized with the C-terminal sequence -Cys-Xaa-Xaa-Xaa (where Xaa is any amino acid) can be posttranslationally modified by proteolytic, lipidation, and methylation reactions. a peptide necessary for mating between haploid a and a cells of the yeast S. cerevisiae. At least three genes have been associated with its posttranslational maturation, which results in a C-terminal S-farnesyl cysteine a-methyl ester residue. The resulting structures,
first found in the peptidyl mating factors from the jelly fungi Tremella mesenterica and Tremella brasiliensis, contain C-terminal cysteine residues that are modified by both an S-isoprenyl group in a thioether linkage and a-methyl esterification. Additional examples include the a mating factor and RAS proteins from Saccharomyces cerevisiae and the mammalian Ras proteins, large G-proteins, cyclic GMP phosphodiesterase and nuclear lamins 3. The mating pheromones of S.pombe, namely, P-factor secreted by h1 cells and M-factor secreted by h2 cells, mediate cell-cell interaction prior to mating. While P-factor is a peptide of 23 amino acids that does not appear to be modified, M-factor is a nonapeptide whose C-terminal cysteine residue is both farnesylated and carboxylmethylated and resembles S. cerevisiae alpha-factor 4.
Mode of Action
A number of proteins, including Ras superfamily GTP-binding proteins, are processed from precursors carrying a CAAX motif at their C termini. C, A, and X in the motif represent, respectively, cysteine, an aliphatic amino acid, and an unspecified amino acid. The precursors undergo three steps of posttranslational modifications at this motif to generate mature proteins. First, the cysteine residue is prenylated, i.e., either farnesylated or geranylgeranylated, and this is followed by removal of the C-terminal three residues, AAX. The new Cterminal residue, prenylated cysteine, is then methylesterified. These modifications are thought to be important to localize the proteins to the membrane. A membrane-associated activity that methylesterifies prenylcysteine has been detected in S. cerevisiae and mammals and a single activity appears to methylesterify both farnesyl cysteine and geranylgeranyl cysteine. Carboxylmethylation is reversible under physiological conditions, raising the interesting possibility that methylation also regulate a biological activity of the target proteins 5,6,7.
Functions
RAS proteins usually undergo endoproteolytic processing by the RCE1 protease and then carboxyl methylation by a unique methyltransferase known as isoprenylcysteine carboxyl methyltransferase (ICMT). Results indicate that the inhibition of these post-prenylation-processing steps — particularly that of ICMT-catalysed methylation — might provide a better approach to the control of cancer-cell proliferation 8.
STE14 gene is required for the methylation of the C-terminal farnesyl cysteine residue in the production of active a factorand other polypeptides 3.
Lipidation reaction, farnesylation of the cysteine residue in the peptide is required for the methyltransferase activity, suggesting that methyl esterification follows the lipidation reaction in the cell 3.
Insulin secretion, a membrane-associated activity that methylesterifies prenylcysteine has been detected in S. cerevisiae and mammals, and a single activity appears to methylesterify both farnesylcysteine and geranylgeranyl cysteine. A link between receptor-mediated signal transduction and carboxylmethylation of Ras superfamily proteins has been suggested to exist in human neutrophils, and carboxylmethylation of Rap1, a member of the family, has been demonstrated in regulated insulin secretion 9.
About the Author
Bio-Synthesis Inc. a leading provider for Custom Antibody, Custom Peptide Synthesis, Custom siRNA Synthesis, Organic Laboratory Technique, Antibiotic Peptides.
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Frequently Asked Questions...
Drummers: What is my drum set worth to sell in online auction? More info..?
If you're a drummer and have experience buying drum kits, please help me come up with a price for all that I have. What would you pay for..
Mapex 5-piece M Series
22" Bass (Hydrolic Heads)
3 toms (10", 12", and 14")
14" snare
Heavey duty mapex symbol stands.
(Sabian B8 series) 14" hi hat symbols, 20" ride, 16" thin crash, 12" splash and Sabain Aax 8" splash with all symbol stands.. All of which are very heavey duty. About the best I could find.
Double Bass pedal (with an extra single bass pedal)
Very pretty maple red shells.
I love my drums but need the money asap. What should I try and get out of them..? Thanks
I got over 1,900 in the whole kit.. Think it'd sell for about 1,000?
I actually paid about $2,400 all together (but another retailer had the drum kit for $733.00 plus shipping) so that screwed me up a little. No big deal
Answer:
i agree with both the other answers. your kit is worth 1000$, but i dont know how many people would be willing to pay. understand that you paid a lot for the hardware. while this is nice, people tend to not care about how heavy duty a stand may be. you're going to get most of the money from the drums. im not sure which would get you more.... selling everything separately or as a whole. but its something to consider. if you live in a big city, you could probably craigslist it for 1000. people are more willing to pay more money if they can physically see what they're buying before they pay for it, ya know? plus, then you dont have to worry about shipping. i know in chicago, there are 50+ drum postings per DAY!!!! anyways, sounds like youre in the ballpark with knowing what its worth. next step is to research a bit and see what people are willing to pay!
